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首 页 > 产品介绍 > 全血DNA提取试剂 |
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Nucleic acid isolation is the most technically demanding and laborious procedure performed in molecular cloning laboratory. As a researcher, if you still separate "buffey coat" from whole blood to separate genomic DNA, your method is out!
A recent developed DNA extraction method can directly use whole blood as the samples. It is the use of magnetic beads covered with nucleic acid–binding matrices.
Whole blood is viscous sample. when we process such complex sample with spin column, the membrane may be clogged because of the cell debris and other precipitate, then result in inefficient recovery of samples. When using Bionewtech® whole blood DNA purification kit, this problem is never happened, because in the liquid phase the nucleic acid in the lysate is specifically adsorbed to the surfaces of magnetic beads but the precipitate in the lysate isn’t adsorbed. Then washing the magnetic beads for 2 times to eliminate the impurities, finally eluting pure DNA from magnetic beads.
Sample: human whole blood,1-30
Method: To evaluate of Bionewtech® whole blood DNA purification kit, Purifying DNA from 30 whole blood withBionewtech® Kit, then runing electrophoresis, testing OD vaule and testing picogreen to evaluate the kit
Result: From the gel picture, we know we obtained the integrate, pure DNA from all 30 samples after purification, and from the OD260/280 picture, we know the pufity is fine to all the 30 samples. So,Bionewtech® Kit is excellent.
Bionew™ 全血DNA提取试剂盒 2010-09-09
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